Coding
nirS

Part:BBa_K4354007:Design

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-22)


nirS nitrite reductase[Pseudomonas stutzeri]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 537
    Illegal PstI site found at 943
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 537
    Illegal PstI site found at 943
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 537
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 537
    Illegal PstI site found at 943
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 537
    Illegal PstI site found at 943
    Illegal NgoMIV site found at 112
    Illegal NgoMIV site found at 304
    Illegal AgeI site found at 691
    Illegal AgeI site found at 1308
    Illegal AgeI site found at 1750
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1060
    Illegal BsaI.rc site found at 1339


Design Notes

By cloning AMO,HAO,NAR,NAR,NOR,NOS to the Bacillus subtilis, We want to make it an engineering strain that could remove the nitrogenous compounds in the waste water.


Source

We search its sequence from NCBI(geneID:10944547) and synthesize it.The organism is Pseudomonas stutzeri.

References

1.Song Qin, Xu Lei.Advancement of Enzymology of Heterotrophic Nitrification.BIOTECHNOLOGY BULLETIN.DOI:10.13560/j.cnki.biotech.bull.1985.2008.05.009 2.Xu Tao.METABOLIC CHARACTERISTICS OF HETEROTROPHIC NITRIFYING-AEROBIC DENITRIFYING STRAIN DIAPHOROBACTER SP.PD-7.Taiyuan University of Technology in China.2015 May10.